NGS Clean-up and Size Selection
High Prep™ PCR 50ml, 500ml
HighPrep™ PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic bead technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation utilizing your current protocol as well as being performed manually.
Ordering Information
50ml High Prep PCR catalogue number AQ 60050
500ml High Prep PCR catalogue number AQ 60500
Features:
High recovery of amplicons greater than 100 bp
Efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants
Stable and high recovery of PCR products post-cleanup
No centrifugation or filtration
Protocol easily customised for amplicon size selection
14-month shelf-life (refrigerated)
Amplicons purified with HighPrep PCR system are ready to be used in the
following applications:
PCR
Mutation detection and genotyping
Sequencing (Sanger and Next Generation)
Microarrays
Restriction enzyme clean up
Cloning
Primer walking
High Recovery of Amplicons
Figure shows PCR products over 50 bp
recovered from a 10µl PCR reaction.
Data were analyzed on an Agilent Tapestation 2200.
For research purposes only. Not for use in diagnostics procedures.