NGS Clean-up and Size Selection 

High Prep™ PCR 50ml, 500ml 

HighPrep™ PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic bead technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation utilizing your current protocol as well as being performed manually. 
 
Ordering Information 
50ml High Prep PCR catalogue number AQ 60050 
500ml High Prep PCR catalogue number AQ 60500 
 
Features: 
High recovery of amplicons greater than 100 bp 
Efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants 
Stable and high recovery of PCR products post-cleanup 
No centrifugation or filtration 
Protocol easily customised for amplicon size selection 
16-month shelf-life (refrigerated) 
Amplicons purified with HighPrep PCR system are ready to be used in the  
following applications: 
 
PCR 
Mutation detection and genotyping 
Sequencing (Sanger and Next Generation) 
Microarrays 
Restriction enzyme clean up 
Cloning 
Primer walking 
High Recovery of Amplicons 
Figure shows PCR products over 50 bp  
recovered from a 10µl PCR reaction. 
Data were analyzed on an Agilent Tapestation 2200. 
For research purposes only. Not for use in diagnostics procedures.